Thermo scientific accessories for fluoroskan ascent fl. Fluorometers fluorescence spectrophotometers a fluorescence spectrophotometer or fluorometer, fluorospectrometer, or fluorescence spectrometer measures the fluorescent light. The instrument is controlled with the flexible thermo scientific ascent software. The whole thermo scientific microplate reader family is compatible with skanit software. Crude and purified proteasome activity assays are affected by. The remaining fitcbsa concentration and the recovery rate percentage were calculated using a calibration curve established with the original solution. Total sample recovery in eppendorf protein lobind conical tubes. A fluoroskan ascent fl microplate fluorometer thermo fisher, 374 fluoroskan ascent fl was used for fluorometric measurements. After unpacking the instrument, place the instrument on a flat. The fluorescence and luminescence were determined using a fluoroskan ascent fl microplate fluorometer and a luminometer thermo. The fluoroskan ascent fl is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric.
It features compatibility with 1 to 384 well plate. Fluoroskan ascent and fluoroskan ascent fl microplate fluorometers, thermo scientific. How do i program the fluoroskan microplate fluorometer or fluoroskan fl microplate. Thermo scientific accessories for fluoroskan ascent fl microplate fluorometer and luminometer perform a range of fluorometric and luminometric research applications with thermo scientific fluoroskan ascent fl, offering fast read speed and topbottom plate reading. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Thermofisher scientific fluoroskan ascent fl fluorometer. Instrument fluoroskan microplate fluorometer 5200110 fluoroskan fl microplate fluorometer and luminometer 5200220 reagents quantit dsdna assay kit, high sensitivity q33120 microplates. Top related posts fluoroskan ascent microplate fluorometer pdf.
The 374 fluoroskan ascent microplate fluorometer features excellent optical performance. Highthroughput identification of peptide agonists against. Comparison of thrombin generation assay with conventional. Fluoroskan fl microplate fluorometer and luminometer. Fluoroskan ascent and fluoroskan ascent fl microplate. Microplate fluorometers for a variety of uses in the life science field, including dna, rna, and enzyme studies, feature enhanced reading speeds. Thermo scientific fluoroskan ascent fl the thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. Fluorimetric analysis was performed with the fluoroskan. Thermo scientific fluoroskan ascent fl from thermo fisher. Fluoroskan ascent fl microplate fluorometer and luminometer. Fluoroskan ascent microplate fluorometer from thermo. May 25, 2019 here my blog where i share pdf files with my readers.
Total sample recovery in eppendorf protein lobind conical. The fluoroskan combines all the advantages of fluorometric techniques with all the convenience of microplate. Thermo labsystems 374 fluoroskan ascent microplate fluorometer. After unpacking the instrument, place the instrument on a flat, level, dry surface. At all times, avoid leaving the switch on if a power failure is anticipated. Plug one end of the supplied power cord into the qubit 2. The fluoroskan ascent fl is a combined highquality microplate fluorometer and luminometer intended for laboratory research use by professional personnel. Skanit software for microplate readers thermo fisher scientific. Toxicological, enzymatic, and molecular assessment. The fluoroskan ascent fl fluoroskan ascent can carry out both top and bottom reading. Fluoroskan fl microplate fluorometer and luminometer, 100240 v, 5060 hz with two dispensers 5200222 includes pc software and filter pairs. Ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as.
After 12 h incubation at 37 c, fluorescence intensity was measured using fluoroskan ascent microplate fluorometer. The highperformance fluorometers in fluoroskan and fluoroskan fl microplate readers make them ideal fluorescence plate readers for life science research applications such as. Download pdf copy thermo scientific fluoroskan ascent microplate fluorometer is a compact and robust instrument with excellent optical performance for a variety of research applications. This microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studie. Ascent microplate fluorometer from thermolabsystems.
Tumor cellderived exosomes home to their cells of origin. Downloaded from the thermo scientific reading room. The direct illumination optics of the fluoroskan ascent produces a highly focused light beam. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic measurements from the very start of the reaction. Pdf fluorescence microplate assay for the detection of oxidative.
Ascent fl fluoroskan ascent can carry out both top and bottom reading. A standard curve of histamine concentrations constructed from the standard histamine provided in the assay kit. Spectral peaks may shift as a function of temperature, ph, solvent, or coupling chemistry. Pdf an easy and efficient fluorescent method for detecting. One of the most commonly used classes of anticancer drugs presently in clinical practice is the platinumbased drugs, including cisplatin. Decreased transcriptioncoupled nucleotide excision repair. Standard factoryinstalled filter pair for fluoroskan and fluoroskan fl instruments. The thermo scientific fluoroskan ascent fl microplate reader. Spectrophotometers and spectrometers fluorescence spectrophotometers. The fluoroskan ascent fl is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric applications even in the same run. Thermo scientific fluoroskan ascent fl microplate fluorometer and luminometer is ideal for life science research applications.
Proteome and allergenome of asian wasp, vespa affinis, venom. Pdf fluorescence microplate assay for the detection of. Used thermo fluoroskan ascent 374 fluorometer for sale. Crude and purified proteasome activity assays are affected. The fluoroskan ascent, a dedicated microplate fluorometer, offers high sensitivity for a wide variety of fluorometric applications. Featuring excellent optical performance, the fluoroskan fl microplate fluorometer and luminometer is ideal for life science fluorometric and luminometric assays. The tg curve was plotted with a fluoroskan ascent microplate fluorometer thermo scientific, vantaa, finland and thrombinoscope software thrombinoscope bv, maastricht, the netherlands, using a reagent with low ppp. Two independent experiments were performed in triplicate n6. Up to three reagent dispensers can be fitted onboard, making the reagent addition simple and highly accurate. Tumor cellderived exosomes home to their cells of origin and. The dss1016d power connector is a standard threepronged connector that supports the power cord.
Standard curve of pla 1 fluorescence intensity excitation at absorbance 505 nm and emission at 515 nm as measured by using a fluoroskan ascent fl microplate fluorometer and luminometer. Aminolevulinic acid mediated photodynamic effect mdpi. Ly fluorescence was measured in a 96well plate with a fluoroskan ascent microplate fluorometer thermo. This table contains only a partial list of the fluorescent dyes that can be used with the fluoroskan and fluoroskan fl microplate fluorometers. We monitored reactions for 1 hour, using a fluoroskan ascent fl microplate fluorometer thermo fisher scientific, tokyo, japan, set at an excitation wavelength of 390 nm and an emission. Thermo scientific accessories for fluoroskan ascent fl microplate fluorometer and luminometer.
Recommended filter pairs for various fluorescent dyes. Each, skanit sw for microplate readers, research edition. Standard curve of pla 1 fluorescence intensity excitation at absorbance 505 nm and emission at 515 nm as measured by using a fluoroskan ascent fl microplate fluorometer and. In fields such as fluoromunassay fia, fluoroskan offers extremely accurate measurements down to 1 pmol. We tested the relative factor xa activity of all venoms using a thermo scientific fluoroskan ascent microplate fluorometer by adding. The fluoroskan ascent from labsystems is a microplate fluorometer which. Reader comparison june 08 specifications for pdf ts labor. Zmwrky79 positively regulates maize phytoalexin biosynthetic. Microplate fluorometers for a variety of uses in the life science field, including dna. Reactive oxygen species produced up or downstream of calcium. Perform a range of fluorometric and luminometric research applications with. Thermo scientific fluoroskan ascent fl microplate fluorometer.
The fluoroselect fluorometer can be powered with 4 aa alkaline batteries or a power adapter 5vdc. Proteome and allergenome of asian wasp, vespa affinis. Pdf watersoluble aldehydes acetaldehyde, propionaldehyde and nonwater soluble. The efficacy of cisplatin therapy is often limited by. Therefore, the assumption that all statin compounds will have the same effect within the. Dedicated fluoroskan microplate fluorometer and dualmode fluoroskan fl fluorometer and luminometer. Please follow these guidelines for setting up the switch. Fluoroskan ascent fl is a microplate fluorometer and luminometer with excellent optical performance for a variety of research applications, offering versatile plate formats, fast reading speed, up to three dispensers and top bottom reading of plates.
The antiinflammatory effects of statins hmgcoa reductase inhibitors within the cardiovascular system are wellestablished. The remaining fitcbsa concentration and the recovery rate percentage were calculated using a calibration curve established with the original. The thermo scientific fluoroskan ascent fl microplate reader is. Download any file posted by another user of a community feature that you know, or. Ssp05374, fluorometeric microplate reader software controlled with ex em filter set, display printer rs232c interface with manual labsystems multiskan ms filters installed vibratory shaking usb ports thermo scientific multiskan. Most fluorescence spectrophotometers feature optional connections to chart recorders or the ability to download data onto a. Crude and purified proteasome activity assays are affected by type of microplate article in analytical biochemistry 4461 october 20 with 51 reads how we measure reads. The tg curve was plotted with a fluoroskan ascent microplate fluorometer thermo scientific, vantaa, finland and thrombinoscope software thrombinoscope bv, maastricht, the. Fluoroskan ascent fl is a microplate fluorometer and luminometer with excellent optical performance for a variety of research applications, offering versatile plate formats, fast reading. The source of the excitation light and the detector of the emission light are both located on the same side of the microplate. Coagulotoxic effects by brown snake pseudonaja and. This table contains only a partial list of the fluorescent dyes that can be used with the fluoroskan.
The fluoroskan combines all the advantages of fluorometric techniques with all the convenience of microplate photometry. Polyhedrocytes in blood clots of type 2 diabetic patients. Six plasmodium species are known to naturally infect humans. It features compatibility with 1 to 384 well plate formats and pcr plates, up to three onboard dispensers, an incubator and a shaker. The fluoroskan ascent fl is a combined highquality microplate fluorometer and luminometer intended for laboratory research use by. Fluorescence of the cells and the media was detected using a fluoroskan ascent fl microplate fluorometer thermo, waltham, ma, usa excitation at 495 nm and emission at 507 nm.
F luoroskan, fluoroskan fl, and l uminoskan microplate readers. Measurement with the fluoroskan ascent fl and fluoroskan ascent occurs in a narrow angle. Coagulotoxic effects by brown snake pseudonaja and taipan. The thermo scientific fluoroskan ascent is a computercontrolled microplate fluorometer for life science research applications, such as fluorometric protein and. The thermo scientific fluoroskan ascent fl microplate reader is a. Nov 22, 2018 fluorescence was measured with excitation at 485 nm and emission at 537 nm in a fluoroskan ascent microplate fluorometer thermo fisher scientific inc. Ly fluorescence was measured in a 96well plate with a fluoroskan ascent microplate fluorometer thermo fisher scientific. Fluoroskan, fluoroskan fl, and luminoskan filterbased. The luminescence was measured with a fluoroskan ascent fl microplate fluorometer and luminometer thermofisher scientific. Technical note fluoroskan microplate fluorometer sensitive fluorometric quantification of dna in microplate format materials cat. The thermo scientific fluoroskan ascent microplate fluorometer and luminoskan ascent microplate luminometer together with white and black thermo scientific. Mixed species infections occur regularly but morphological discrimination by microscopy is difficult and multiplicity of. Statins reduce lipopolysaccharideinduced cytokine and.
Fluorescence microplate assay for the detection of oxidative burst products in tobacco cell suspensions using 2. The time required for the emitted light to reach 1e of its initial intensity, where e is the naperian base 2. The decrease in scopoletin fluorescence was measured in a microplate fluorometer fluoroskan ascent cf. Fluorometers fluorescence spectrophotometers a fluorescence spectrophotometer or fluorometer, fluorospectrometer, or fluorescence spectrometer measures the fluorescent light emitted from a sample at different wavelengths, after illumination with a xenon flash lamp. Cancer exosome homing to the parent cell line in vivo 100 l of dmem, containing 2. Fluorescence was measured with excitation at 485 nm and emission at 537 nm in a fluoroskan ascent microplate fluorometer thermo fisher scientific inc. The versatile assay programming of the fluoroskan ascent fl model allows fast measurements of fluorometric gfp and luminometric luciferase from the same well. Promoters of zmtps1 and zmksl5 were also cloned and ligated into pcambia3301gus to replace the 35s promoter. After a flash of onoff welcome screen, similar to the one shown in figure 1, the screen automatically. Thermo fisher scientific the 374 fluoroskan ascent microplate fluorometer features excellent optical.
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