Dec 21, 2019 download citation on researchgate actinobacillus seminis isolated from cattle the organism commonly referred to as actinobacillus. Clinical and pathological changes in rams experimentally. Actinobacillus actinomycetemcomitans is a typical cause of periodontitis but it may also be related to systemic infections and arterial plaques. Emended description of porcine pasteurella aerogenes, pasteurella mairii and actinobacillus rossii. In commercial flocks semen examination is only worthwhile if there is some doubt as to ram fertility. Speciesspecific multiplex pcr for the diagnosis of brucella.
Pdf actinobacillus seminis as a cause of abortion in a uk. It was not until 1960 that baynes and simmons 1960 reported another organism which could be included in the genus. Actinobacillus seminis definition of actinobacillus. Files are available under licenses specified on their description page. It was first isolated in the united kingdom in 1991 and in one survey was found to be present in the semen of 19% of infertile rams. Method to grow actinobacillus pleuropneumoniae biofilm on. Actinobacillus m a taxonomic genus within the family pasteurellaceae several gramnegative bacteria, some of which are pathogens. The organisms can also gain entry through breaks in the skin. Actinobacillus seminis as a cause of ram infertility in kenya. Bacterial respiratory diseases mannheimia haemolytica biotype a, pasteurella trehalosi biotype t, mycoplasma ovipneumoniae iii. The actinobacillus group shares many biological properties with the genus pasteurella.
This study aimed to investigate clinical and pathological changes associated with experimental infections with a. Infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. Frequency of outbreaks increased with the industrialization of swine production. Small ruminant research elsevier small ruminant research 21 1996 227231 actinobacillus seminis as a cause of ram infertility in kenya k mbaia, s. Pdf sheep epididymitis by actinobacillus seminis in the state of. Seminis disease guides provide descriptions and pictures of the more commonly found diseases and disorders worldwide by species. Actinobacilli are usually transmitted via the aerosol route or by close contact. Actinobacillus seminis infection in sheep in the republic of south africa. All structured data from the file and property namespaces is available under the creative commons cc0 license. Clinical infections are usually unresponsive to treatment, and because infected animals are of considerable financial value, economic losses. Actinobacillus lignieresi article about actinobacillus.
A model for demonstrating the adhesion of actinobacillus seminis to epithelial cells. Pdf this study reports the occurrence of sheep epididymitis and the isolation of actinobacillus seminis in the state of pernambuco, brazil. Summary eighteen strains of actinobacillus seminis isolated from ovine semen samples and epididymitis were characterized according to their cultural and. Actinobacillus seminis an overview sciencedirect topics. Dear sir, in a paper submitted for publication in the australian veterinary journal, we have reported serological evidence of actinohocilluj seminis infection in 23 ewes from a border leicester flock. Actinobacillus seminis is a common cause of ovine epididymitis and ram infertility throughout the world 2, 6, 10, 21. Semen examination does have a role in artificial insemination programs or in flocks where single sire joining groups are used.
Infected rams were found to have abscessed testes and epididymi and. Mbiuki department of clinical studies, university of nairobi, nairobi, kenya b department of veterinary pathology, microbiology and parasitology, university of nairobi, nairobi, kenya accepted 29 november. Overview of actinobacillosis generalized conditions. An outbreak of epididymitis and orchitis associated with actinobacillus seminis infection is reported. Actinobacillus seminis infection in sheep in the republic. Evaluation of actinobacillus pleuropneumoniae diagnostic tests using samples from experimentally infected pigs by giordana souza costa dr.
Experimentally infected with actinobacillus seminis and histophilus. Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of a. Actinobacillus seminis is a gramnegative bacterium of the pasteurellaceae family that is involved in ovine epididymitis. However, the most common causative agents of these infections are brucella ovis, actinobacillus seminis, and histophilus somni. Actinobacillus definition, any of several spherical or rodshaped, aerobic, parasitic bacteria of the genus actinobacillus, certain species of which, as a. Actinobacillus seminis in sheep, australian veterinary. It is a gramnegative, nicotinamide adenine dinucleotide nadindependent, non.
Clinical and pathological changes in rams experimentally infected. Actinobacillus seminis as a cause of abortion in a uk. Actinobacillus suis an overview sciencedirect topics. Actinobacillus suis can cause similar disease and lesions but is not as contagious as actinobacillus pleuropneumoniae app. Actinobacillus suislike organism how is actinobacillus. It has high morbidity and mortality rates and can cause severe economic losses to swine producers. List of bacteria and similar organisms presenting at the. Isolated actinobacillus actinomycetemcomitans from periodontitis patients releases leukotoxin which kills t cells by some pathways 10. Speciesspecific multiplex pcr for the diagnosis of brucella ovis, actinobacillus seminis, and histophilus somni infection in rams by valeria s moustacas, teane ma silva, luciana f costa, mariana n xavier, custodio a carvalho, erica a costa, tatiane a paixao and renato l santos. On the other hand, one of the haemophilus agni strains studied hoerlein strain m65043 was included in the species actinobacillus seminis dna binding value, 91%.
The first page of the pdf of this article appears above. Clinical infections are usually unresponsive to treatment, and because infected animals are of considerable financial value. Actinobacillus pleuropneumoniae disease and serology. Actinobacillus seminis definition of actinobacillus seminis. Colonies are small, sticky and adherent to the agar surface. A study for the differentiation of actinobacillusseminis. Abstract the organism commonly referred to as actinobacillus seminis is an accepted cause of suppurative epididymitis of rams worldwide, it has also been associated with polyarthritis, myocarditis and posthitis in sheep, and isolated from aborting ewes, goat does and their foetuses. Actinobacillus seminis was isolated in pure and mixed culture from the urethra in two yearlings, one which was infected and one which was not, while bacilli were isolated from one uninfected ram. In the outbreak, both young unmated rams and mature breeding rams were affected. Actinobacillosis refers to a group of diseases caused by gramnegative coccobacilli in the genus actinobacillus. Overview of actinobacillosis generalized conditions merck. In the united states, app was first reported in 1957. Although there are 22 different bacterial species in this genus, only four a pleuropneumoniae, a suis, a equuli, and a lignieresii are frequently associated with disease in animals.
Actinobacillus seminis infection of the head of epididymis of a 4yearold suffolk ram. Four of the rams had abnormal semen and three were also infertile. Identification of an immunogenic protein of actinobacillus seminis that is present in microvesicles. Actinobacillus pleuropneumoniae is a gramnegative bacterium and a member of the pasteurellaceae family. Evidence from numerical taxonomic analysis and dnadna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella.
Speciesspecific multiplex pcr for the diagnosis of brucella ovis. The organisms are capable of infecting cattle and other domestic animals and, rarely, are associated with human infection. Misdiagnosis of the disease as actinobacillus pleuropneumoniae app infection or other septicemias likely contributes to underreporting of the true prevalence of the disease. For each disease and disorder, you will find the common name, the cause, where it occurs, symptoms, and conditions necessary for development and control measures. Read serological comparison between histophilus ovis, actinobacillus seminis and brucella ovis, australian veterinary journal on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. This bacterium is the causative agent of porcine pleuropneumonia, which is a highly contagious respiratory disease causing important economical losses to the worldwide pig industry. Actinobacillus pleuropneumoniae app causes respiratory disease in swine throughout the world. Actinobacillus actinobacillus actinomycetemcomitans refer to haemophilus actinomycetemcomitans.
Actinobacillus seminis as a cause of abortion in a uk sheep flock article pdf available in the veterinary record 14417. Actinobacillus ureae article about actinobacillus ureae. Actinobacillus capsulatus synonyms, actinobacillus capsulatus pronunciation, actinobacillus capsulatus translation, english dictionary definition of actinobacillus capsulatus. Pdf actinobacillus seminis as a cause of abortion in a. Actinobacillus is a genus of gramnegative, nonmotile and nonsporeforming, oval to rodshaped bacteria occurring as parasites or pathogens in mammals, birds, and reptiles. Actinobacillus pleuropneumoniae app iowa state university. The aim of this study was to develop a multiplex pcr assay for simultaneous detection of brucella ovis, actinobacillus seminis, and. Pdf clinical and pathological changes in rams experimentally. Most species are nonhemolytic on blood agar, however colonies of actinobacillus suis on sheep blood agar is always surrounded by a clearly marked zone of hemolysis. The most common causative agents of these infections are brucella ovis, actinobacillus seminis, and histophilus somni.
Pdf sheep epididymitis by actinobacillus seminis in the. Isolation of actinobacillus seminis from a goat with. Actinobacillus definition of actinobacillus by the free. Actinobacillus seminis infection in rams constitutes a spectrum of. Semen obtained from infected rams yielded actinobacillus seminis in pure or mixed culture in five of the six clinically infected rams. Viral respiratory diseases pi3, rsv, adenovirus, reovirus, enzootic nasal tumors, opp ii. A model for demonstrating the adhesion of actinobacillus. Fertility testing of rams nsw department of primary. Actinobacillus seminis in sheep actinobacillus seminis in sheep baynes, i. The isolates of a seminis showed similar phenotypic profiles and electrophoretic protein patterns to the type strain of a seminis but were distinct from histophilus ovis previously isolated from rams with epididymitis in scotland. Actinobacillus seminis 2 pv actinobacillus succinogenes 2 pv actinobacillus suis 2 pv actinobacillus ureae 2 old name pasteurella ureae actinobaculum actinobaculum schaalii 2.
Murray pr, baron ej, jorgensen jh, pfaller ma, tenover fc, yolken hy eds 2. Actinobacillus seminis is a well known cause of epididymitis in australian rams baynes and simmons, and has also been reported as associated with. This was actinobacillus seminis isolated from epididymitis in three rams in australia. Actinobacillus seminis, campylobacter fetus, escherichia coli, histophilus somni, mannheimia haemolytica, proteus vulgaris and gram. Actinobacillus capsulatus definition of actinobacillus. The main causative agents of ovine infectious epididymitis. Speciesspecific multiplex pcr for the diagnosis of. Pdf infectious epididymitis is considered a major cause of economic losses. Mar 21, 20 infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. Linear integrated circuits by ramakant gaikwad pdf opamps and linear integrated circuits on amazon. December 2008 h a species abiotrophia 2 abiotrophia defectiva actinobacillus 2 actinobacillus capsulatus 2 actinobacillus equuli 2 actinobacillus hominis 2 actinobacillus lignieresii 2 actinobacillus pleuropneumoniae actinobacillus rossii 2 actinobacillus seminis 2 actinobacillus suis 2 actinobacillus urea actinobacillus. Rapid and accurate identification of human isolates of pasteurella and related species by. The bacteria are facultatively anaerobic or aerobic, capable of fermenting carbohydrates without production of gas, and of reducing nitrates. By using welldefined techniques under optimum conditions it is possible adequately to define the biochemical characteristics of typical a.
Morphology, biochemical reactions, pigmentation, antigens, and cell envelope proteins were examined in 12 strains of haemophilus somnus, haemophilus agni, histophilus ovis, and actinobacillus seminis. Actinobacillus seminis was isolated from the semen of five rams on four farms. The aim of this study was to develop a multiplex pcr assay for simultaneous detection of brucella ovis, actinobacillus seminis, and histophilus. Actinobacillus equuli definition of actinobacillus equuli. A genus of gramnegative, immotile and nonsporeforming, oval to rodshaped, often pleomorphic bacteria which occur as parasites or pathogens in mammals.
Isolation of actinobacillus seminis from rams in the. Morphological, biochemical, antigenic, and cytochemical. Identification of an immunogenic protein of actinobacillus. Actinobacillus seminis isolated from cattle sciquest. The diagnosis of epididymitis by actinobacillus seminis was confirmed with association of the clinical findings. The species is not recognised today and is almost certainly what we now term haemophilus somnus. Pcr methods for rapid identification and characterization. The present study reports the first isolation of actinobacillus seminis from a goat in brazil. Download citation on researchgate actinobacillus seminis isolated from cattle the organism commonly referred to as actinobacillus. It is associated with epididymitis of sheep references. In immunodiffusion tests, hh group bacteria had at least two. Deoxyribonucleic acid relationships of histophilus ovis.
This short communication describes a retrospective analysis of bacterial isolates obtained between 1994 and 1996 in south wales from ejaculates of 63 rams that were considered by. Twenty rams of age 18 to 24 months were infected by intraepididymal inoculation of a. Actinobacillus seminis as a cause of ram infertility in. M c healey, h h hwang, y y elsner, and a v johnston department of animal, dairy and veterinary sciences, college of agriculture, utah state university, logan 843225600. Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. The etiologic agent, actinobacillus suis, is only now being studied in detail. Sep 10, 2010 actinobacillus actinomycetemcomitans is a typical cause of periodontitis but it may also be related to systemic infections and arterial plaques. Simone oliveira, advisor chair department of veterinary medicine of population abstract the early detection of pigs subclinically infected with a.
Actinobacillusspecies and their role in animal disease. Isolation and characterization of actinobacillus seminis strains from. So far, only low levels of genetic relatedness with actinobacillus seminis and currently recognized members of the family pasteurellaceae have been detected. Actinobacillus equuli actinobacillus ligniersii actinobacillus pleuropneumoniae. Twentyfour isolates of actinobacillus seminis were typed by pcr ribotyping, repetitive. The major pathogenic actinobacillus species in veterinary medicine are presented in table 20. Infected with actinobacillus seminis and histophilus somni. Actinobacillus seminis as a cause of abortion in a uk sheep flock.
These pathogen safety data sheets, regulated under workplace hazardous materials information system whmis legislation, are produced for personnel working in the life sciences as quick safety reference material relating to infectious microorganisms. Since then, the prevalence of ovine epididymitis due to a. Adobe acrobat xi pro was used to ocr the text and also for the merging and conversion to the final presentation pdf format. Actinobacillus ross, actinobacillus equuli, and actinobacillus suis have been reported in cases of metritis, abortion, low number of viable pigs at birth, and decreased litter weights.
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